7MN0
N74D mutant of the HIV-1 capsid protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2015-10-15 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.000030 |
| Spacegroup name | P 6 |
| Unit cell lengths | 91.741, 91.741, 57.163 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.870 - 2.900 |
| R-factor | 0.2222 |
| Rwork | 0.219 |
| R-free | 0.27360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xfx |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.904 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.870 | 45.870 | 3.080 |
| High resolution limit [Å] | 2.900 | 8.710 | 2.900 |
| Rmerge | 0.123 | 0.034 | 1.134 |
| Rmeas | 0.129 | 0.036 | 1.191 |
| Rpim | 0.039 | 0.011 | 0.364 |
| Total number of observations | 69089 | 2539 | 10578 |
| Number of reflections | 6190 | 247 | 999 |
| <I/σ(I)> | 16.7 | 63.6 | 2.1 |
| Completeness [%] | 99.8 | 98.7 | 99.4 |
| Redundancy | 11.2 | 10.3 | 10.6 |
| CC(1/2) | 0.999 | 1.000 | 0.694 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 3350, NaI, Sodium Cacodylate, Glycerol |
