7MLJ

Crystal structure of Thermus thermophilus reiterative transcription complex with 4nt oligo-G RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	186.309, 104.656, 298.413
Unit cell angles	90.00, 98.12, 90.00

Refinement procedure

Resolution	46.210 - 3.750
R-factor	0.266
Rwork	0.265
R-free	0.28330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g7h
RMSD bond length	0.002
RMSD bond angle	0.515
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.810
High resolution limit [Å]	3.750	3.750
Rmerge	0.103	1.031
Number of reflections	57607	2910
<I/σ(I)>	14.522
Completeness [%]	97.9	99
Redundancy	4.2	4.2
CC(1/2)		0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	295	0.1 M Tris-HCl pH8.4, 180 mM potassium chloride, 50 mM Magnesium chloride, 9% (m/v) PEG 4000