7MLB
Crystal structure of Thermus thermophilus transcription initiation complex with 5nt RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97907 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 186.172, 104.266, 299.017 |
Unit cell angles | 90.00, 97.95, 90.00 |
Refinement procedure
Resolution | 44.620 - 3.600 |
R-factor | 0.2341 |
Rwork | 0.233 |
R-free | 0.26970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g7h |
RMSD bond length | 0.002 |
RMSD bond angle | 0.534 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.660 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.116 | 0.845 |
Number of reflections | 66110 | 3055 |
<I/σ(I)> | 10.733 | |
Completeness [%] | 98.5 | 92.3 |
Redundancy | 4 | 3.3 |
CC(1/2) | 0.624 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 295 | 0.1 M Tris-HCl pH8.4, 180 mM potassium chloride, 50 mM Magnesium chloride, 9% (m/v) PEG 4000 |