7ML5

Structure of the Starch Branching Enzyme I (BEI) complexed with maltododecaose from Oryza sativa L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-02
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	47.670, 80.107, 182.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.970 - 2.350
R-factor	0.1682
Rwork	0.165
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vu2
RMSD bond length	0.003
RMSD bond angle	0.613
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.970	2.434
High resolution limit [Å]	2.350	2.350
Rmerge	0.091	0.316
Number of reflections	28616	2155
<I/σ(I)>	14.7
Completeness [%]	95.2	73.45
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	300	30% PEG8K, 550 mM sodium acetate, and 100 mM sodium cacodylate (pH 6.9)