7MK3
Crystal structure of NPR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 78.980, 78.980, 395.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.420 - 3.060 |
| R-factor | 0.2454 |
| Rwork | 0.244 |
| R-free | 0.26980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7mk2 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.438 |
| Data reduction software | XDS (Built=20200417) |
| Data scaling software | XSCALE (Built=20200417) |
| Phasing software | PHASER (dev_3922) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.470 | 3.170 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Number of reflections | 24848 | 2429 |
| <I/σ(I)> | 21.56 | |
| Completeness [%] | 99.8 | |
| Redundancy | 42.7 | |
| CC(1/2) | 0.998 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 60 mM HEPES, pH 7.5, 9.5% PEG3350, 0.125 M tri-sodium citrate, 75 mM sodium chloride, 0.5 mM TCEP |
