7MJY
MiaB in the complex with s-adenosyl-L-homocysteine and RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0033 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.834, 79.982, 110.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.370 - 1.860 |
| R-factor | 0.1758 |
| Rwork | 0.174 |
| R-free | 0.20880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qgq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.292 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.890 |
| High resolution limit [Å] | 1.860 | 5.050 | 1.860 |
| Rmerge | 0.152 | 0.064 | 0.918 |
| Rmeas | 0.163 | 0.069 | 1.014 |
| Rpim | 0.058 | 0.024 | 0.421 |
| Total number of observations | 303282 | ||
| Number of reflections | 40640 | 2224 | 1971 |
| <I/σ(I)> | 4.8 | ||
| Completeness [%] | 100.0 | 100 | 99.6 |
| Redundancy | 7.5 | 7.5 | 5.1 |
| CC(1/2) | 0.997 | 0.681 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM sodium cacodylate, pH 5.5, 13% PEG 4000, and 3% 1,6-hexanediol |
