7MJF
Crystal structure of Candidatus Liberibacter solanacearum dihydrodipicolinate synthase with pyruvate and succinic semi-aldehyde bound in active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2018-12-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953723 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.101, 132.881, 155.062 |
| Unit cell angles | 90.00, 99.96, 90.00 |
Refinement procedure
| Resolution | 45.100 - 2.200 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7loy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.916 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.812 | 1.990 |
| High resolution limit [Å] | 1.925 | 1.930 |
| Number of reflections | 195500 | |
| <I/σ(I)> | 8.32 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% w/v PEG3000, 0.1 M sodium citrate, pH 5.5 |
