7MIA

Maize rayado fino virus protease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2019-03-31
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.541870
Spacegroup name	P 1 21 1
Unit cell lengths	43.197, 73.255, 54.336
Unit cell angles	90.00, 90.94, 90.00

Refinement procedure

Resolution	30.900 - 1.900
R-factor	0.2058
Rwork	0.203
R-free	0.23810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a5u
RMSD bond length	0.006
RMSD bond angle	0.835
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.900	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	27471	2663
<I/σ(I)>	7.03
Completeness [%]	99.4
Redundancy	1.9
CC(1/2)	0.990	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	100 mM BIS-TRIS Propane (pH 7.5), 200 mM sodium acetate and 20 % PEG 3350