7MI2

Seb1-G476S RNA binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	111.649, 46.307, 32.296
Unit cell angles	90.00, 99.00, 90.00

Refinement procedure

Resolution	42.700 - 1.400
R-factor	0.1722
Rwork	0.171
R-free	0.18800
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5mdu
RMSD bond length	0.013
RMSD bond angle	1.343
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Rmeas		0.979
Rpim	0.030
Number of reflections	29365	744
<I/σ(I)>	25.9
Completeness [%]	91.2	47.5
Redundancy	3.9
CC(1/2)	0.994	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2M KCl, 0.025M MgSO4, 20% (v/v) PEG200, 0.05 M HEPES-Na, pH 7.0