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7MHX

KcsA E71V closed gate with Ba2+

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-E
Synchrotron siteAPS
Beamline24-ID-E
Temperature [K]98
Detector technologyPIXEL
Collection date2019-12-03
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.9792
Spacegroup nameI 4
Unit cell lengths155.169, 155.169, 75.340
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution50.010 - 2.850
R-factor0.20414
Rwork0.202
R-free0.24416
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1k4c
RMSD bond length0.010
RMSD bond angle1.900
Data reduction softwareiMOSFLM
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0267)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]77.5803.000
High resolution limit [Å]2.8502.850
Number of reflections210763082
<I/σ(I)>9
Completeness [%]99.8
Redundancy6
CC(1/2)0.9200.140
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP29350 mM sodium acetate pH 5.5, 50 mM magnesium acetate, 25% PEG 400

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