7MHX
KcsA E71V closed gate with Ba2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 98 |
Detector technology | PIXEL |
Collection date | 2019-12-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | I 4 |
Unit cell lengths | 155.169, 155.169, 75.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.850 |
R-factor | 0.20414 |
Rwork | 0.202 |
R-free | 0.24416 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4c |
RMSD bond length | 0.010 |
RMSD bond angle | 1.900 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.580 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 21076 | 3082 |
<I/σ(I)> | 9 | |
Completeness [%] | 99.8 | |
Redundancy | 6 | |
CC(1/2) | 0.920 | 0.140 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM sodium acetate pH 5.5, 50 mM magnesium acetate, 25% PEG 400 |