7MHX
KcsA E71V closed gate with Ba2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2019-12-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 4 |
| Unit cell lengths | 155.169, 155.169, 75.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.850 |
| R-factor | 0.20414 |
| Rwork | 0.202 |
| R-free | 0.24416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.900 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.580 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 21076 | 3082 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6 | |
| CC(1/2) | 0.920 | 0.140 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM sodium acetate pH 5.5, 50 mM magnesium acetate, 25% PEG 400 |
