7MHR

KcsA E71V closed gate with K+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	98
Detector technology	PIXEL
Collection date	2019-10-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	I 4
Unit cell lengths	155.832, 155.832, 76.255
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.770
R-factor	0.1692
Rwork	0.168
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4c
RMSD bond length	0.015
RMSD bond angle	2.095
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.920
High resolution limit [Å]	2.770	2.770
Number of reflections	23491	3370
<I/σ(I)>	10.3
Completeness [%]	99.7
Redundancy	6.6
CC(1/2)	0.977	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM sodium acetate pH 5.5, 50 mM magnesium acetate, 25% PEG 400