7MG1
Concanavalin A crystallized without DNA glycoconjugate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-07-19 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 61.190, 85.180, 89.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.620 - 2.000 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jbc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.516 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.620 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.112 | 0.440 |
| Number of reflections | 14120 | 1906 |
| <I/σ(I)> | 3.199 | |
| Completeness [%] | 92.2 | 82.4 |
| Redundancy | 7.12 | 6.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | Sitting drop comprising 1 uL of Concanavalin A (32 uM) + 1 uL crystallization condition (Helix screen, condition H3: 0.1 M potassium chloride, 0.005 M barium chloride dihydrate, 0.05 M bis-Tris (pH 7), 12% w/v PEG 8000). Reservoir contained 70 uL of crystallization condition. |
