7MFB

Crystal structure of antibody 10E8v4 Fab - light chain H31F variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2020-03-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	141.800, 56.231, 69.261
Unit cell angles	90.00, 100.85, 90.00

Refinement procedure

Resolution	40.040 - 1.740
R-factor	0.2237
Rwork	0.223
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iq9
RMSD bond length	0.012
RMSD bond angle	1.315
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.780
High resolution limit [Å]	1.740	4.750	1.750
Rmerge	0.100	0.042	0.596
Rmeas	0.118	0.050	0.680
Rpim	0.061	0.027	0.316
Number of reflections	48111	2173	2394
<I/σ(I)>	5.6
Completeness [%]	88.2	76.7	87.3
Redundancy	3.3	3	3.4
CC(1/2)		0.994	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	12% PEG3350, 20% iso-propanol, 0.1M Tris-HCl 8.5