7MFA

Crystal structure of antibody 10E8v4-P100fA+P100gA Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-07-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	95.778, 53.795, 328.528
Unit cell angles	90.00, 90.60, 90.00

Refinement procedure

Resolution	35.010 - 2.400
R-factor	0.2392
Rwork	0.238
R-free	0.28610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iq9
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.390	6.510	2.400
Rmerge	0.152	0.058	2.628
Rmeas	0.170	0.065	3.079
Rpim	0.073	0.029	1.549
Number of reflections	108309	5807	4232
<I/σ(I)>	6.2
Completeness [%]	81.4	82.9	63.7
Redundancy	4.4	4.4	2.4
CC(1/2)		0.995	0.109

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	10% PEG 8000, 5% Iso-propanol, 0.2M Ammonium sulfate 0.1M HEPES 7.4