7MEK
Structure of copper bound to Ycnl
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-13 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.3776 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 90.190, 90.190, 209.120 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.520 - 2.110 |
R-factor | 0.1968 |
Rwork | 0.195 |
R-free | 0.21750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3esm |
RMSD bond length | 0.002 |
RMSD bond angle | 0.526 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.160 |
High resolution limit [Å] | 2.110 | 2.110 |
Rmeas | 0.138 | |
Number of reflections | 54321 | 3960 |
<I/σ(I)> | 9.64 | 2.64 |
Completeness [%] | 99.6 | 98 |
Redundancy | 5.3 | |
CC(1/2) | 0.995 | 0.569 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M citric acid, 2.17 M ammonium sulfate |