7ME5
Structure of the extracellular WNT-binding module in Drl-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-24 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 56.978, 91.583, 76.362 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.970 - 2.000 |
R-factor | 0.2315 |
Rwork | 0.230 |
R-free | 0.25270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ygn |
RMSD bond length | 0.003 |
RMSD bond angle | 0.625 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3915) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.790 | 45.790 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.067 | 0.020 | 0.898 |
Rmeas | 0.073 | 0.022 | 1.048 |
Rpim | 0.028 | 0.009 | 0.524 |
Total number of observations | 90856 | 1060 | 3366 |
Number of reflections | 13786 | 186 | 929 |
<I/σ(I)> | 19.1 | 75.6 | 1.5 |
Completeness [%] | 99.5 | 98.8 | 94.2 |
Redundancy | 6.6 | 5.7 | 3.6 |
CC(1/2) | 0.999 | 0.999 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294.15 | 12 mg/ml protein, 100mM Tris (ph 8.5), 100 mM sodium acetate, 25% PEG 6000, 15% glycerol |