7ME5

Structure of the extracellular WNT-binding module in Drl-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-24
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	56.978, 91.583, 76.362
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.970 - 2.000
R-factor	0.2315
Rwork	0.230
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ygn
RMSD bond length	0.003
RMSD bond angle	0.625
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (dev_3915)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.790	45.790	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.067	0.020	0.898
Rmeas	0.073	0.022	1.048
Rpim	0.028	0.009	0.524
Total number of observations	90856	1060	3366
Number of reflections	13786	186	929
<I/σ(I)>	19.1	75.6	1.5
Completeness [%]	99.5	98.8	94.2
Redundancy	6.6	5.7	3.6
CC(1/2)	0.999	0.999	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294.15	12 mg/ml protein, 100mM Tris (ph 8.5), 100 mM sodium acetate, 25% PEG 6000, 15% glycerol