7ME3
YfeA oligomer crystal 3, form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-24 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.283 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.090, 75.850, 106.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.450 - 2.250 |
R-factor | 0.1689 |
Rwork | 0.166 |
R-free | 0.21780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uxs |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.500 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 52503 | 5090 |
<I/σ(I)> | 7.3 | 4.32 |
Completeness [%] | 99.5 | |
Redundancy | 2 | |
CC(1/2) | 0.968 | 0.654 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293.15 | 32% PEG 4000, 20 mM Bis-Tris propane, pH 6.3, 50 mM sodium chloride, 0.05%(w/v) sodium azide, 10 mM manganese chloride |