7ME2
YfeA oligomer crystal 2, form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-24 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.74 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.310, 76.430, 107.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.510 - 1.850 |
R-factor | 0.1538 |
Rwork | 0.152 |
R-free | 0.18390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uxs |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.510 | 1.916 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 89302 | 6433 |
<I/σ(I)> | 12.67 | 3.22 |
Completeness [%] | 94.9 | |
Redundancy | 2 | |
CC(1/2) | 0.998 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293.15 | 32% PEG 4000, 20 mM Bis-Tris propane, pH 6.3, 50 mM sodium chloride, 0.05%(w/v) sodium azide, 10 mM manganese chloride |