7MDJ

The structure of KcsA in complex with a synthetic Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	I 4
Unit cell lengths	138.409, 138.409, 71.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.750
R-factor	0.2044
Rwork	0.201
R-free	0.26490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6w0i
RMSD bond length	0.008
RMSD bond angle	1.753
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.750
High resolution limit [Å]	2.700	2.700
Number of reflections	17621	17621
<I/σ(I)>	11.3
Completeness [%]	99.9
Redundancy	6.2
CC(1/2)	0.990	0.200

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	50 mM sodium acetate/50 mM ammonium acetate, 50 mM magnesium acetate, 25% PEG 400.