7MDH

STRUCTURAL BASIS FOR LIGHT ACITVATION OF A CHLOROPLAST ENZYME. THE STRUCTURE OF SORGHUM NADP-MALATE DEHYDROGENASE IN ITS OXIDIZED FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-06
Detector	MAR scanner 345 mm plate
Spacegroup name	C 2 2 21
Unit cell lengths	145.424, 153.944, 160.042
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.221 *
Rwork	0.221
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bmd
RMSD bond length	0.249
RMSD bond angle	22.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.300	2.400
Rmerge	0.068 *
Number of reflections	66459
<I/σ(I)>	10.8
Completeness [%]	94.9	99.8
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	recombinant E.coli	0.02 (mM)
2	1	1	dithiothreitol	10 (mM)