7MCY
Crystal structure of Staphylococcus aureus Cystathionine gamma lyase, Holoenzyme with bound NL3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-10 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.92013 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 105.448, 105.448, 287.772 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.710 - 1.720 |
R-factor | 0.1584 |
Rwork | 0.158 |
R-free | 0.16410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ixz |
RMSD bond length | 0.007 |
RMSD bond angle | 0.992 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.750 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmerge | 0.117 | 0.846 |
Rpim | 0.031 | 0.288 |
Number of reflections | 85730 | 3829 |
<I/σ(I)> | 20.3 | 1.4 |
Completeness [%] | 99.4 | 89.8 |
Redundancy | 14.4 | 6.3 |
CC(1/2) | 0.998 | 0.826 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M Tris-HCl, pH 7.5, 1.4 M Tri Sodium Citrate |