7MCU
Crystal structure of Staphylococcus aureus Cystathionine gamma lyase, Holoenzyme with bound NL2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.91956 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 104.890, 104.890, 288.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.940 - 2.400 |
R-factor | 0.1595 |
Rwork | 0.157 |
R-free | 0.19380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ixz |
RMSD bond length | 0.007 |
RMSD bond angle | 0.941 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.142 | 0.361 |
Rpim | 0.042 | 0.142 |
Number of reflections | 30864 | 1723 |
<I/σ(I)> | 2.2 | 0.4 |
Completeness [%] | 97.4 | 77.3 |
Redundancy | 11.3 | 5.4 |
CC(1/2) | 0.993 | 0.919 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 291 | 0.1 M Tris-HCl, pH 7.6, 1.3 M Tri Sodium Citrate |