7MCT

Crystal structure of Staphylococcus aureus Cystathionine gamma lyase, Holoenzyme with bound NL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9189
Spacegroup name	I 41 2 2
Unit cell lengths	105.115, 105.115, 288.104
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.710 - 1.600
R-factor	0.162
Rwork	0.162
R-free	0.17200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ixz
RMSD bond length	0.006
RMSD bond angle	0.832
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.114	2.558
Rpim	0.025	0.585
Number of reflections	106360	7458
<I/σ(I)>	19.9	1.4
Completeness [%]	99.6	95.5
Redundancy	22.1	19.4
CC(1/2)	1.000	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	291	0.1 M Tris-HCl, pH 7.6, 1.4 M Tri Sodium Citrate