7MCN
Crystal structure of Staphylococcus aureus Cystathionine gamma-lyase, Holoenzyme with High HEPES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-05 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.91956 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 104.282, 104.282, 287.676 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.600 - 2.520 |
R-factor | 0.166 |
Rwork | 0.164 |
R-free | 0.18910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ixz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.904 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.580 |
High resolution limit [Å] | 2.520 | 2.520 |
Rmerge | 0.152 | 0.997 |
Rpim | 0.033 | 0.211 |
Number of reflections | 27451 | 1944 |
<I/σ(I)> | 19.3 | 4 |
Completeness [%] | 99.8 | |
Redundancy | 22.6 | |
CC(1/2) | 0.999 | 0.979 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M HEPES-Na, pH 7.5, 1.3 M Tri sodium Citrate |