7MCL
Crystal structure of Staphylococcus aureus Cystathionine gamma-lyase, PLP bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.91956 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 104.966, 104.966, 289.569 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.960 - 2.400 |
R-factor | 0.1834 |
Rwork | 0.181 |
R-free | 0.21440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ixz |
RMSD bond length | 0.008 |
RMSD bond angle | 0.984 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.450 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.194 | |
Number of reflections | 31961 | 1811 |
<I/σ(I)> | 7 | |
Completeness [%] | 99.9 | 98.3 |
Redundancy | 10.5 | 6.6 |
CC(1/2) | 0.996 | 0.608 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 291 | 0.1 M Tris-HCl pH 7.6, 1.4 M Tri sodium Citrate |