7MCB
Crystal structure of Staphylococcus aureus Cystathionine gamma lyase Holoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-04 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.92010 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 105.133, 105.133, 288.148 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.390 - 2.140 |
R-factor | 0.163 |
Rwork | 0.162 |
R-free | 0.18380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ixz |
RMSD bond length | 0.007 |
RMSD bond angle | 0.849 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.190 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.099 | |
Number of reflections | 45133 | 3211 |
<I/σ(I)> | 23.7 | |
Completeness [%] | 99.8 | 97.5 |
Redundancy | 18.7 | 18.1 |
CC(1/2) | 0.999 | 0.955 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 291 | 0.1 M HEPES , pH 7.6 1.4 M Tri sodium Citrate |