7MC4
Crystal structure of a single-chain E/F type bilin lyase-isomerase MpeQ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-03-03 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.389, 173.213, 83.818 |
Unit cell angles | 90.00, 126.02, 90.00 |
Refinement procedure
Resolution | 19.999 - 2.500 |
R-factor | 0.2345 |
Rwork | 0.232 |
R-free | 0.28530 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.013 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 51398 | 2424 |
<I/σ(I)> | 3.4 | |
Completeness [%] | 99.8 | |
Redundancy | 3.7 | |
CC(1/2) | 0.977 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Protein concentration: 5 mg/mL, 2.1 M DL-Malic acid |