7MBK

N-terminal domain of mouse surfactant protein B, 6W mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.28270
Spacegroup name	P 21 21 21
Unit cell lengths	38.482, 60.251, 76.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.450 - 2.170
R-factor	0.2215
Rwork	0.220
R-free	0.23890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vyn
RMSD bond length	0.015
RMSD bond angle	1.869
Data reduction software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.450	2.290
High resolution limit [Å]	2.170	2.170
Rmeas	0.147	0.148
Number of reflections	9974	375
<I/σ(I)>	23.1
Completeness [%]	96.6
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	0.1 M ammonium sulfate, 0.05 M magnesium sulfate, 0.1 M sodium citrate, 16.5 % PEG smear medium