7M92

Crystal structure of unknown function protein protein B9J08_000055 Candida auris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97890
Spacegroup name	P 21 21 21
Unit cell lengths	52.263, 111.925, 124.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.170 - 2.350
R-factor	0.2013
Rwork	0.199
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q7g
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.390
High resolution limit [Å]	2.350	6.370	2.350
Rmerge	0.098	0.083	1.000
Rmeas	0.107	0.091	1.122
Rpim	0.043	0.037	0.500
Total number of observations	187954
Number of reflections	30716	1711	1369
<I/σ(I)>	10.2
Completeness [%]	99.0	99.8	89.9
Redundancy	6.1	5.8	4.6
CC(1/2)		0.988	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1M Sodium Citrate pH5.0 , 30% Jeffamine ED-2001 pH7.0