7M80
Human DNA Pol eta with dA-ended primer and dATP: in crystallo reaction for 100 s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-26 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 61 |
Unit cell lengths | 98.844, 98.844, 82.037 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.100 - 1.980 |
R-factor | 0.1612 |
Rwork | 0.157 |
R-free | 0.21420 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4ecq |
RMSD bond length | 0.008 |
RMSD bond angle | 0.980 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.300 | 32.300 | 2.010 |
High resolution limit [Å] | 1.980 | 5.370 | 1.980 |
Rmerge | 0.079 | 0.024 | 0.567 |
Total number of observations | 139977 | ||
Number of reflections | 31730 | 1611 | 1571 |
<I/σ(I)> | 8.8 | ||
Completeness [%] | 99.7 | 97.8 | 100 |
Redundancy | 4.4 | 4.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 295 | 15% PEG 2000 MME, 100 mM MES |