7M7Z
Human DNA Pol eta with dA-ended primer and dATP: in crystallo reaction for 40 s
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 |
| Unit cell lengths | 98.779, 98.779, 82.064 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.080 - 1.820 |
| R-factor | 0.184 |
| Rwork | 0.180 |
| R-free | 0.22790 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4ecq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.322 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 1.860 |
| High resolution limit [Å] | 1.820 | 4.960 | 1.830 |
| Rmerge | 0.118 | 0.037 | 0.680 |
| Number of reflections | 40321 | 2037 | 2007 |
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 99.6 | 97.5 | 100 |
| Redundancy | 5.4 | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 295 | 15% PEG 2000 MME, 100 mM MES |
