7M7Z
Human DNA Pol eta with dA-ended primer and dATP: in crystallo reaction for 40 s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 61 |
Unit cell lengths | 98.779, 98.779, 82.064 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.080 - 1.820 |
R-factor | 0.184 |
Rwork | 0.180 |
R-free | 0.22790 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4ecq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.322 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.860 |
High resolution limit [Å] | 1.820 | 4.960 | 1.830 |
Rmerge | 0.118 | 0.037 | 0.680 |
Number of reflections | 40321 | 2037 | 2007 |
<I/σ(I)> | 10.1 | ||
Completeness [%] | 99.6 | 97.5 | 100 |
Redundancy | 5.4 | 5.4 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 295 | 15% PEG 2000 MME, 100 mM MES |