7M7O

Human DNA Pol eta with dT-ended primer and 0.1 mM dAMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.589, 98.589, 82.196
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.040 - 1.800
R-factor	0.1829
Rwork	0.180
R-free	0.22360
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4ecq
RMSD bond length	0.012
RMSD bond angle	1.404
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.095	0.032	0.718
Total number of observations	158373
Number of reflections	38173	1826	1956
<I/σ(I)>	9.2
Completeness [%]	90.6	84	93.3
Redundancy	4.1	4.4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	295	15% PEG 2000 MME, 100 mM MES