7M7M

Human DNA Pol eta with rA-ended primer and dAMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.444, 98.444, 82.105
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.310 - 1.460
R-factor	0.183
Rwork	0.181
R-free	0.21130
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4ecq
RMSD bond length	0.007
RMSD bond angle	1.187
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.490
High resolution limit [Å]	1.460	3.960	1.460
Rmerge	0.059	0.028	0.565
Total number of observations	220433
Number of reflections	76542	3796	3133
<I/σ(I)>	16.6
Completeness [%]	97.7	94.4	80.3
Redundancy	2.9	3	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	295	15% PEG 2000 MME, 100 mM MES