7M7L
Human DNA Pol eta with dA-ended primer and dAMPNPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 |
| Unit cell lengths | 98.645, 98.645, 81.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.040 - 1.580 |
| R-factor | 0.178 |
| Rwork | 0.175 |
| R-free | 0.21320 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4ecq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.921 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.040 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.069 | 0.477 |
| Number of reflections | 62075 | 6106 |
| <I/σ(I)> | 11.7 | 2.9 |
| Completeness [%] | 99.7 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 295 | 15% PEG 3350, 100 mM MES |
