7M5I

Endolysin from Escherichia coli O157:H7 phage FAHEc1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	110
Detector technology	PIXEL
Collection date	2019-08-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.954
Spacegroup name	P 32 2 1
Unit cell lengths	67.874, 67.874, 134.437
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.850 - 1.710
R-factor	0.17503
Rwork	0.173
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ET6A
RMSD bond length	0.014
RMSD bond angle	1.731
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.850	1.740
High resolution limit [Å]	1.710	1.710
Number of reflections	39675	2064
<I/σ(I)>	13.7
Completeness [%]	99.9	98
Redundancy	1	1
CC(1/2)	0.999	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Sodium Chloride, 1.6 M Ammonium sulfate, 0.1 M Sodium HEPES pH 7.5