7M5F
Contact-dependent inhibition system from Serratia marcescens BWH57
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-14 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.691, 97.241, 44.495 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.640 - 1.590 |
R-factor | 0.1417 |
Rwork | 0.140 |
R-free | 0.16670 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.909 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (dev_3409) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.620 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmerge | 0.132 | 0.650 |
Number of reflections | 35744 | 1750 |
<I/σ(I)> | 20.82 | 2.98 |
Completeness [%] | 99.0 | 99.6 |
Redundancy | 6.6 | 4.2 |
CC(1/2) | 0.775 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 8% v/v tacsimate pH 4.0, 20% w/v PEG 3350, cryo glycerol 15%, trypsin treated |