7M57
Crystallographic structure of a primitive orthorhombic crystal form of STMV
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 1998-06-15 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 192.080, 202.290, 177.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.000 - 4.000 |
Rwork | 0.200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nia |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 4.250 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.085 | 0.280 |
Number of reflections | 48422 | 865 |
<I/σ(I)> | 5.6 | 1.2 |
Completeness [%] | 81.8 | 36 |
Redundancy | 6.8 | 2.3 |
CC(1/2) | 0.940 | 0.430 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | Crystals were grown by sitting drop vapor diffusion in Cryschem plates using 0.6 ml reservoirs. The drops were 6 to 8 ul and composed of equal volumes of a 5 mg/ml virus stock solution buffered with 0.1 M phosphate at pH 6.5, and the reservoir. The reservoirs were 16% ammonium sulfate buffered at pH 6.0 with 0.1 M phosphate. Crystallization was a room temperature. |