7M55

B6 Fab fragment bound to the MERS-CoV spike stem helix peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-02-07
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.977410
Spacegroup name	C 1 2 1
Unit cell lengths	93.589, 60.444, 79.710
Unit cell angles	90.00, 93.75, 90.00

Refinement procedure

Resolution	43.490 - 1.400
R-factor	0.1745
Rwork	0.173
R-free	0.19960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nb8
RMSD bond length	0.009
RMSD bond angle	1.086
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.490	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	86876	8539
<I/σ(I)>	12.71	1.51
Completeness [%]	99.4	98.26
Redundancy	1.9	1.9
CC(1/2)	1.000	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Magnesium Chloride and 20% (w/v) PEG3350