7M4L

DNA Polymerase Lambda, TTPaS:At Mn2+ Product State Ternary Complex, 60 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	55.970, 62.753, 140.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.029 - 1.701
R-factor	0.1906
Rwork	0.189
R-free	0.21530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.070	0.062	0.715
Rmeas	0.077	0.068	0.792
Rpim	0.030	0.027	0.333
Total number of observations	343935
Number of reflections	53587	2959	2225
<I/σ(I)>	9.2
Completeness [%]	97.4	98.7	82.6
Redundancy	6.4	6.1	5
CC(1/2)		0.994	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG