7M4K

DNA Polymerase Lambda, TTPaS:At Ca2+ Ground State Ternary Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	56.001, 62.507, 140.191
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.117 - 1.719
R-factor	0.197
Rwork	0.196
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.750
High resolution limit [Å]	1.719	4.670	1.720
Rmerge	0.106	0.124	0.437
Rmeas	0.117	0.137	0.490
Rpim	0.049	0.059	0.218
Number of reflections	52546	2779	2396
<I/σ(I)>	10.7
Completeness [%]	98.9	96.5	90.4
Redundancy	5.7	5.3	4.6
CC(1/2)		0.972	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG