7M4J

DNA Polymerase Lambda, dCTP:At Mn2+ Product State Ternary Complex, 960 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	55.975, 63.660, 139.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.036 - 2.377
R-factor	0.2273
Rwork	0.226
R-free	0.26120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.377	6.510	2.400
Rmerge	0.065	0.043	0.620
Rmeas	0.081	0.053	0.856
Rpim	0.047	0.031	0.589
Number of reflections	32603	1017	818
<I/σ(I)>	12.1
Completeness [%]	94.7	90.5	84.1
Redundancy	2.4	2.6	1.7
CC(1/2)		0.995	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG