7M4E

DNA Polymerase Lambda, dCTP:At Mg2+ Reaction State Ternary Complex, 120 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	55.825, 62.088, 142.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.219 - 1.903
R-factor	0.2098
Rwork	0.209
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.081	0.074	0.960
Rmeas	0.090	0.083	1.070
Rpim	0.038	0.035	0.464
Total number of observations	235495
Number of reflections	39411	2070	1899
<I/σ(I)>	10.8
Completeness [%]	99.4	95.3	96
Redundancy	6	5.4	5.1
CC(1/2)		0.991	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG