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7M4A

DNA Polymerase Lambda, TTP:At Mn2+ Product State Ternary Complex, 20 min

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-ID
Synchrotron siteAPS
Beamline22-ID
Temperature [K]100
Detector technologyCCD
Collection date2016-04-22
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)1.00
Spacegroup nameP 21 21 21
Unit cell lengths56.050, 62.727, 140.405
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution31.174 - 1.868
R-factor0.1891
Rwork0.188
R-free0.22020
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3upq
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareHKL-3000
Refinement softwarePHENIX (v1.15.2-3472)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]50.00050.0001.900
High resolution limit [Å]1.8685.0701.870
Rmerge0.0780.0710.899
Rmeas0.0850.0780.983
Rpim0.0320.0320.394
Number of reflections4184522702026
<I/σ(I)>10.5
Completeness [%]99.998.7100
Redundancy76.25.9
CC(1/2)0.9920.836
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.527780-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG

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