7M4A
DNA Polymerase Lambda, TTP:At Mn2+ Product State Ternary Complex, 20 min
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.050, 62.727, 140.405 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.174 - 1.868 |
R-factor | 0.1891 |
Rwork | 0.188 |
R-free | 0.22020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3upq |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (v1.15.2-3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.900 |
High resolution limit [Å] | 1.868 | 5.070 | 1.870 |
Rmerge | 0.078 | 0.071 | 0.899 |
Rmeas | 0.085 | 0.078 | 0.983 |
Rpim | 0.032 | 0.032 | 0.394 |
Number of reflections | 41845 | 2270 | 2026 |
<I/σ(I)> | 10.5 | ||
Completeness [%] | 99.9 | 98.7 | 100 |
Redundancy | 7 | 6.2 | 5.9 |
CC(1/2) | 0.992 | 0.836 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG |