7M4A

DNA Polymerase Lambda, TTP:At Mn2+ Product State Ternary Complex, 20 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	56.050, 62.727, 140.405
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.174 - 1.868
R-factor	0.1891
Rwork	0.188
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.900
High resolution limit [Å]	1.868	5.070	1.870
Rmerge	0.078	0.071	0.899
Rmeas	0.085	0.078	0.983
Rpim	0.032	0.032	0.394
Number of reflections	41845	2270	2026
<I/σ(I)>	10.5
Completeness [%]	99.9	98.7	100
Redundancy	7	6.2	5.9
CC(1/2)		0.992	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG