7M47

DNA Polymerase Lambda, TTP:At Mg2+ Product State Ternary Complex, 60 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-22
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	56.049, 62.462, 140.059
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.839 - 1.648
R-factor	0.181
Rwork	0.180
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.680
High resolution limit [Å]	1.648	4.480	1.650
Rmerge	0.068	0.056	0.517
Rmeas	0.074	0.062	0.566
Rpim	0.028	0.025	0.225
Number of reflections	59768	3221	2663
<I/σ(I)>	10.8
Completeness [%]	99.3	98.6	91.6
Redundancy	7.1	6.5	5.7
CC(1/2)		0.995	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG