7M43
DNA Polymerase Lambda, TTP:At Ca2+ Ground State Ternary Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-31 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.988, 62.653, 140.386 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.023 - 1.978 |
R-factor | 0.1944 |
Rwork | 0.193 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3upq |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (v1.15.2-3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.010 |
High resolution limit [Å] | 1.978 | 5.370 | 1.980 |
Rmerge | 0.070 | 0.035 | 0.503 |
Rmeas | 0.076 | 0.038 | 0.598 |
Rpim | 0.029 | 0.015 | 0.317 |
Number of reflections | 35088 | 1918 | 1618 |
<I/σ(I)> | 10.6 | ||
Completeness [%] | 99.3 | 98 | 92.8 |
Redundancy | 6.5 | 6.7 | 3.2 |
CC(1/2) | 0.999 | 0.752 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG |