7M3R
Crystallographic Structure of the Rhombohedral Crystal Form of STMV Grown from Bromide
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2002-06-16 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 166.491, 166.491, 435.839 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.080 - 2.100 |
R-factor | 0.1805 |
Rwork | 0.171 |
R-free | 0.20820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4oq8 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.481 |
Data reduction software | d*TREK |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.160 | 0.320 |
Number of reflections | 200162 | 13000 |
<I/σ(I)> | 4.5 | 2.7 |
Completeness [%] | 86.0 | 35 |
Redundancy | 4.6 | |
CC(1/2) | 0.987 | 0.440 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 279 | Crystals were grown by sitting drop vapor diffusion in Cryschem plates with 0.6 ml reservoirs and 6 to 8 ul drops. The drops were equal amounts of a 5 mg/ml virus stock solution buffered at pH 6.5 with 0.1 M phosphate. The reservoirs were 5% w/v NaBr in 0.1 M phosphate at pH 6.0. Crystallization was at 4 degrees C and the time of development was about 10 days. |