7M2V
Crystallographic Structure of the Rhombohedral Crystal Form of STMV Grown from Chloride
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2002-06-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 165.634, 165.634, 433.315 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
Rwork | 0.205 |
R-free | 0.21680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4oq8 |
Data reduction software | d*TREK |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.300 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.080 | 0.146 |
Number of reflections | 345430 | 9879 |
<I/σ(I)> | 13.4 | 2.7 |
Completeness [%] | 84.0 | |
Redundancy | 4.5 | |
CC(1/2) | 0.998 | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 279 | Crystals grown by sitting drop vapor diffusion in Cryschem plates with 0.6 ml reservoirs and 6 to 8 ul drops. Drops were equal amounts of a 5 mg/ml virus stock solution buffered at pH 6.5 with 0.1 M phosphate, and the reservoir solution. The reservoir solution was 4% w/v NaCl in 0.1 M phosphate at pH 6.0. Crystallization was at 4 degrees C. |