7M2J

Structural Snapshots of Intermediates in the Gating of a K+ Channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	93
Detector technology	CCD
Collection date	2018-08-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	I 4
Unit cell lengths	134.813, 134.813, 72.152
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.632 - 3.201
R-factor	0.2534
Rwork	0.247
R-free	0.30480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vk6
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.660	3.510
High resolution limit [Å]	3.200	3.200
Number of reflections	10837	2566
<I/σ(I)>	8.8
Completeness [%]	100.0
Redundancy	7.4
CC(1/2)	0.990	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	33% PEG400, 100 mM MES, pH 6.5, 100 mM calcium chloride