7M2H
Structural Snapshots of Intermediates in the Gating of a K+ Channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.04 |
Spacegroup name | P 4 |
Unit cell lengths | 154.321, 154.321, 74.242 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.801 - 2.642 |
R-factor | 0.2379 |
Rwork | 0.237 |
R-free | 0.26750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4c |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.801 | 2.730 |
High resolution limit [Å] | 2.640 | 2.640 |
Number of reflections | 54531 | 4451 |
<I/σ(I)> | 9.1 | 1 |
Completeness [%] | 99.9 | |
Redundancy | 6.9 | |
CC(1/2) | 0.990 | 0.430 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 293 | 50 mM MES, pH 6.25, 28% PEG400, 50 mM magnesium acetate |