7M1N
Wild-type Hydrogenobacter thermophilus ferredoxin 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-16 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 39.635, 69.103, 95.788 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 2.060 |
| R-factor | 0.19465925904 |
| Rwork | 0.193 |
| R-free | 0.21295 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iqz |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.758 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.130 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Number of reflections | 15621 | 2921 |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 98.9 | 88.9 |
| Redundancy | 6.6 | 2.1 |
| CC(1/2) | 0.980 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 79% saturated ammonium sulfate, 0.1 M MES (pH 6.5) |
